A clustering analysis using the profiles of 18 loci with limited variability established clear phylogenetic relationships among IST clonal groups. The phylogenetic pattern for the closely related isolates determined using MLVA profiles of 8 highly variable loci was in agreement with that determined using the 26-locus profiles. MLVA data for the 703 IST1 isolates revealed that diversification among the closely related isolates was attributed mainly to four highly variable loci. MLVA was carried out using 26 VNTR loci with a range of degrees of variability. The global phylogenetic patterns determined by IST, PFGE, and MLVA were concordant. Miranda NG Portable is available for immediate download from the Miranda NG Portable homepage.A panel of 916 isolates, including 703 closely related IST1 isolates, were characterized by inter-IS1 spacer typing (IST), pulsed-field gel electrophoresis (PFGE), and multilocus variable-number tandem-repeat (VNTR) analysis (MLVA) to evaluate the utility of MLVA as a molecular tool for the phylogenetic analysis of Shigella sonnei.
And it's in Format, so it automatically works with the Platform including the Menu and Backup Utility. It supports upgrades by installing right over an existing copy, preserving all settings.
Miranda NG Portable is packaged in a Installer so it will automatically detect an existing installation when your drive is plugged in. The interface and setup is a bit more involved than other IM clients like Pidgin Portable, but the customization options available are richer. Functionality can be greatly enhanced with an extensive set of plugins including Skype and more. It supports ICQ, AIM, MSN, Jabber, Yahoo, IRC and more. Miranda IM uses very little memory and is extremely fast. Miranda NG is a multi protocol instant messenger client for Windows. Update automatically or install from the portable app store in the Platform. And it's open source and completely free. It's packaged in Format so it can easily integrate with the Platform. Very light on system resources and extremely fast. Miranda NG is an enhanced version of the popular multi-protocol instant messaging client for Windows - Miranda IM. A new version of Miranda NG Portable has been released.
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